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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Bioscience Jurnal Biologi Universitas Andalas Jurnal Riset Kimia Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Hilirisasi IPTEKS (JHI) Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Bioscientist : Jurnal Ilmiah Biologi
Anthoni Agustien
Jurusan Biologi, FMIPA Universitas Andalas Padang
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Jurnal Biologi Universitas Andalas

 1   2 
Isolasi, Karakterisasi dan Potensi Bakteri Endofitik dari Tanaman Zodia (Evodia suaveolens Scheff) sebagai Penghasil Antibiotika Melisa Ainil Fajri; Anthoni Agustien; Periadnadi Periadnadi
Jurnal Biologi Universitas Andalas Vol 4, No 2 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.2.%p.2015

Abstract

Endophytic bacterium are important to produce antibiotic. We have experimentally studied zodia plant (Evodia suaveolens Scheff) in attempting to isolate and characterize the bacterium. We have also examined potentiality of each isolate to produce antibiotic. We obtained three a isolate of  endophytic bacterium that produce antibiotic compounds. Among three isolates, two could form high inhibition zone. MZS-01 formed 10.4 mm inhibition zone against Eschericia coli, while MZS-03 formed 6.96 mm inhibition zone against Staphylococcus aureus. We described the isolates MZS-01 and MZS-03 based on macroscopis and microscopis characteristic of colony  culture.
Kondisi Bakteriologis Air Sumur di Sekitar Tempat Pembuangan Akhir Air Dingin Kota Padang Cici Wulandari; Nasril Nasir; Anthoni Agustien
Jurnal Biologi Universitas Andalas Vol 3, No 4 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.4.%p.2014

Abstract

A research on bacteriological condition of ground well water at Air Dingin, Padang was conducted from January to March 2013 at the Health Laboratory Agency (UPTD Balai Laboratorium Kesehatan) of West Sumatra. The water samples were collected by using purposive method at three sampling points in distances of 250 m, 350 m and 450 m from garbage disposal locations. Bacteriological test was done using Most Probable Number method (MPN) with combination of 3:3:3. Escherichia coli was described using indole, methyl red, voges proskauer, citrate (IMViC test). The result showed that MPN of Escherichia coli of ground well water for each distance of 250 m, 350 m and 450 m ranges from 0 cell/100 ml to 2400 cell/100 ml. Based on bacteriological parameter test, quality of the ground well water around the landfill area was not appropriate to be consumed by people, except for ground well water was located at 450 m from landfill.Key words: ground well water, Escherichia coli, landfill, qualilty.
Uji Aktivitas Antibakteri Ekstrak Kental Tanaman Pisang Kepok Kuning (Musa paradisiaca Linn.) terhadap Staphylococcus aureus dan Escherichia coli Ayu Putri Ningsih; - Nurmiati; Anthoni Agustien
Jurnal Biologi Universitas Andalas Vol 2, No 3 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.3.%p.2013

Abstract

Antibacterial activity of crude extracts of pisang kepok kuning plants (Musa paradisiaca Linn.) were tested against Staphylococcus aureus and Escherichia coli. Roots, corms, pseudoterms, flowers, and ripe fruits of pisang kepok kuning cultivar were macerated with ethanol 96% and evaporated in a rotary evaporator at 50C. All of crude extracts were screened for its antibacterial activity by using Agar Well Diffusion Method with concentration based on 200 grams of fresh weight. This research used Completed Randomized Design (CRD) with nested pattern, four replications, and consist of two factors. First factor was bacteria and the second factor was crude extracts of pisang kepok kuning such as roots, corms, pseudoterms, flowers, and ripe fruits. The results showed that all of crude extracts have significant antibacterial activity against S. aureus and E. coli. Crude extract of the corm has highest antibacterial activity against S. aureus with diameter of inhibition area was 20,391 mm with inhibition zone type isirradical and diameter inhibition area 18,962 mm for E. coli that has radical zone.Keywords : Musa paradisiaca Linn., S. aureus, E. coli, antibacterial
Karakterisasi Bakteri Termofilik Penghasil Enzim Protease Netral Widia Firliani; Anthoni Agustien; Fuji Astuti Febria
Jurnal Biologi Universitas Andalas Vol 4, No 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.1.%p.2015

Abstract

A study on the characterization of thermophilic bacteria in producing neutral protease enzyme has been conducted in the Laboratory of Microbiology, Biology Department, Andalas University from October 2013 to March 2014. The bacteria were isolated from a hot spring at Sungai Medang, Kerinci. Water samples  were collected purposively based on gradient temperature at the hot spring which ranged from 450C to 880C. Colony of the bacteria were inoculated using nutrient agar (NA). We obtained 10 isolates of neutral protease enzyme from the thermophilic bacteria with proteolytic index ranged from 0.27 to 3.89. The MIV-14 isolate has the highest proteolytic index which collected from 830C of the hot spring. Characterization of macroscopic, microscopic and biochemical tests were reported. Generally, the colonies have round shape, wavy edge, white, producing endospores and motile. Keywords: characterization, neutral protease, thermophilic bacteria.
Optimasi Parsial Isolat Termofilik M5-24 dalam Produksi Protease Aghni Aznia; Anthoni Agustien; Nasril Nasir
Jurnal Biologi Universitas Andalas Vol 3, No 3 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.3.%p.2014

Abstract

Research about partial optimization of thermophilic bacteria isolate M5-24 on protease production was conducted from May 2013 to December 2013 in Laboratory of Microbiology, Biology Department, Andalas University, Padang. Purpose of the research was to find out optimum condition of protease production by isolate M5-24. This study consisted of three steps. Step 1: Investigating the effect of temperature and pH used completely randomized design in factorial with two replications.The first factor was temperature (450C, 500C, 550C, 600C), the second factor was pH (7, 8, 9, 10). Step 2: Investigating the effect of inoculum doses and inducers used completely randomized design in factorial with three replications. The first factor was inoculum doses (1%, 5%, 10%) and the second factor was inducer (yeast extract 0,5%; pepton 0,1%;  kasein 1%; meat extract 0,5%). While Step 3: Investigating the effect of agitation (125 rpm, 150 rpm, 175 rpm, 200 rpm) used completely randomized design with six replications. The result showed that the best duration of growing was at 18th hours after incubation, the highest enzyme activity was at temperature 500 C;  medium’s pH at 8; the best of inoculum dose was at 5%; the best  inducer was casein 1% and optimum agitation was at 175 rpm. Extrinsic optimization can increase protease production of  thermophilic isolate M5-24 up to 1.7 times.Keywords : Optimization, Protease,  thermophilic, bacteria
Uji Antimikroba Curcuma spp. Terhadap Pertumbuhan Candida albicans, Staphylococcus aureus dan Escherichia coli Rahmi Adila; - Nurmiati; Anthoni Agustien
Jurnal Biologi Universitas Andalas Vol 2, No 1 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.1.%p.2013

Abstract

A study about the effect of fresh extract of Curcuma spp on the growth of Candida albicans, Staphylococcus aureus and Escherichia coli was conducted from March to July 2012 at Microbiology Laboratory, Faculty of Sciences Andalas University. This experiment used nested completely randommized design. The result showed that fresh extracts of Curcuma spp. have different growth inhibition effects on C. albicans, S. aureus dan E. Coli. Temulawak (Curcuma xhantorrhiza) has the highest inhibition effect to the growth of C. albicans (13.07 mm), S. aureus (15.75 mm) and E. coli (31.56 mm). The lowest bactericidal consentration of fresh extract rhizome of temulawak for E. coli were 12.5% and 25%, but there is no inhibition effect to the C. albicans and S. aureus.Keywords: Curcuma, antimicrobial, extract, pathogen microbial
Pengaruh Faktor Abiotik terhadap Produksi Protease dari Isolat Bakteri M1-23 Mira Rosnawita; Anthoni Agustien; Nasril Nasir
Jurnal Biologi Universitas Andalas Vol 4, No 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.1.%p.2015

Abstract

A study on the influence of abiotic factors in producing protease by bacterial isolates M1-23 was conducted from May 2013 to December 2013 in the  Laboratory of Microbiology, Department of  Biology, Andalas University, Padang. This study aimed to find optimal conditions by isolate M1-23 to produce protease. This study consists of two stages. First stage used to produce the best protease and the second used to increase enzim homogenity. The result showed that the highest enzyme activity was  at medium temperature 550 C and pH 7.5. The best homogenity was produced at 125 rpm of agitation process.
Penapisan dan Karakterisasi Bakteri Amilo-Termofilik dari Sumber Air Panas Semurup, Kerinci, Jambi Wira Hastuti; Anthoni Agustien; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 1, No 2 (2012)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.1.2.%p.2012

Abstract

The screening and characterization of amylo-thermophylic bacteria that produced amylase from Semurup hot spring in Kerinci, Jambi was conducted from May to July 2012. The aim of this study was to find out the isolate of amylo-thermophylic bacteria and to analyze amylase activities. This study used experimental methods. This study found 31 isolates of amylo-thermophylic bacteria with the amylolytic index more than 2. The isolate SII- 1.7 has the highest enzyme activity. The form of colony was circular margin, smooth shiny surface and pigmentation cream. The bacterium was gram negative, bacilli cell, positive catalase, non-motile, and has proteolytic and amylolytic activities.Keywords: amylolytic, bacterium, characterization, hot spring, thermophylic
Isolasi dan Karakterisasi Bakteri Alkali-Proteolitik Sumber Air Panas Semurup Kerinci Jambi Yurike Nova Edlin; Anthoni Agustien; Djong Hon Tjong
Jurnal Biologi Universitas Andalas Vol 3, No 4 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.4.%p.2014

Abstract

Thermophile protease has economic value because this enzyme is useful on all kind of industry that used high temperature on it production process. In the effort of obtaining indigenous alkaline thermostable a protease bacterial that produce we explore potential hot spring at Semurup, Kerinci Jambi. This study was conducted from September 2013 to February 2014, Water samples were collected by purposive sampling, the character of thermophilic bacteria were described for each in laboratory of microbiology and biomolecular, development of biology, faculty of mathematic and natural science Andalas university, Padang. There were 40 thermophile colonies, 7 isolates showed alkali proteolytic  index range from 0.3 to 2.00. SIII-10 isolate showed the highest in alkali proteolitic index. The characteristic of SIII-10 are: whute and bolt colony, gram negative, motil, positive sitrat agar and positive gelatin.Key words, Isolation, characterization, alkaline protease, thermostable, Hot spring.
Isolasi Dan Karakterisasi Bakteri Termo-Proteolitik Sumber Air Panas Sungai Medang, Sungai Penuh, Jambi Dina Wahyuna; Anthoni Agustien; - Periadnadi
Jurnal Biologi Universitas Andalas Vol 1, No 2 (2012)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.1.2.%p.2012

Abstract

A study on isolation and characterization of thermoproteolytic bacteria from hot springs at Sungai Medang, Sungai Penuh, Jambi was conducted from March to June 2012 using a descriptive method. Seventy thermophylic bacteria were isolated from the hot springs. A Total of 39 thermoproteolytic bacteria 39 were identified. The highest enzymatic activity (13.59 U/ml) was shown by isolate MI2.3 from a location at which the temperature was 600C. The isolate MI2.3 was characterized as an aerobic thermoalkaliphylic (optimum temperature of 500C, optimum pH of 9.0) bacterium with the ability to produce amylase but neither lipase nor cellulase.Keywords : alkaliphylic, bacterium, enzymatic activity, proteolytic, thermophylic.
Co-Authors - Nurmiati - Nurmiati - Nurmiati Aghni Aznia Akmal Djamaan Armaleni Armaleni Ayu Putri Ningsih Chairul Chairul Cici Wulandari Desma Yuni Deswati Deswati Devi Syafriyani Dianty Putri Dina Wahyuna Djong Hon Tjong Efrizal Efrizal Efrizal Efrizal Fadhila Cantika Ferry Lismanto Syaiful Feskaharny Alamsjah Fuji Astuti Febria Ikel Fitri Yanis Ilsa Septia Putri Inayati Rahmi Irdawati Irdawati Irfan Mustafa irfan mustafa Jetty Nurhajati Kustiasih Lestari Linar Z. Udin Lola Alviche Melisa Ainil Fajri Mira Rosnawita Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nurhajati, Jetty Nurmiati Nurmiati Nurmiati Nurmiati Periadnadi Periadnadi Pingkan Aditiawati PINGKAN ADITIAWATI Rahmadani Eka Pitri Rahmi Adila Riski Budi Yani Rusnam Rusnam Suwirmen, Suwirmen Syamsuardi Syamsuardi Syukria Ikhsan Zam Syukria Ikhsan Zam Tesri Maideliza Tri Widia Ningsih Alam Uci Mela Sari Udin, Linar Z. Widia Firliani Wira Hastuti Yetria Rilda Yurike Nova Edlin Zakaria, Indra Junadi Zuhri Syam Zuhri Syam